P. aeruginosa exotoxin A

Exotoxin A is a tripartate toxin which

  1. binds receptors on the surface of target cells,
  2. through a complex pathway, translocates an enzymatic fragment into the cell cytosol,
  3. inhibits protein synthesis through ADP-ribosylation of polypeptide elongation factor 2 (eEF-2).

The toxin has three structural domains; each domain is responsible for one of the three activities.

Exotoxin A

Selected publications

V. S. Allured, R. J. Collier, S. F. Carroll & D. B. McKay (1986) "Structure of Exotoxin A of Pseudomonas aeruginosa at 3.0 Angstrom Resolution"
Proc. Natl. Acad. Sci. USA 83, 1320-1324. [PubMed]

B. J. Brandhuber, V. S. Allured, T. G. Falbel & D. B. McKay (1988) "Mapping the Enzymatic Active Site of Pseudomonas aeruginosa Exotoxin A"
Proteins 3, 146-154. [PubMed]

J. E. Wedekind, C. B. Trame, M. Dorywalska, P. Koehl, T. M. Raschke, M. McKee, D. FitzGerald, R. J. Collier & D. B. McKay (2001).
"Refined crystallographic structure of Pseudomonas aeruginosa exotoxin A and its implications for the molecular mechanism of toxicity"
J. Mol. Biol. 314, 823-837.
[PubMed] [pdf] [wildtype coordinates] [W218A coordinates]

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